Astrocyte DNA damage and response upon acute exposure to ethanol and corticosterone

• DOI: 10.3389/ftox.2023.1277047
• Publication Date: 2024-01-08T04:35:35Z
• Authors: Ana Laura Reyes-Á...; Magdalena Álvarez...; Silvia Olivera-Bravo; María Vittoria Di...
• Keywords: 0303 health sciences; Ethanol; corticosterone; astrocytes; Toxicology; DDR; Intercellular communication; 03 medical and health sciences; Astrocytes; RA1190-1270; Toxicology. Poisons; intercellular communication; DNA damage; ethanol; Corticosterone

Introduction: Astrocytes are the glial cells responsible for brain homeostasis, but if injured, they could damage neural even deadly. Genetic damage, DNA response (DDR), and its downstream cascades dramatic events poorly studied in astrocytes. Hypothesis methods: We propose that 1 h of 400 mmol/L ethanol and/or μmol/L corticosterone exposure cultured hippocampal astrocytes damages DNA, activating DDR eliciting functional changes. Immunolabeling against γH2AX (chromatin sites), cyclin D1 (cell cycle control), nuclear (base excision repair, BER), cytoplasmic (anti-inflammatory functions) APE1, ribosomal nucleolus proteins together with GFAP S100β plus scanning electron microscopy studies astrocyte surface were carried out. Results: Data obtained indicate significant immediate cell arrest, BER activation. Changes signals number, membrane-attached vesicles strongly suggest a reactivity like without morphological Discussion: Obtained results uncover genome vulnerability activation, might part, be signaled through extracellular vesicles, evidencing complex influence may have on CNS upon short-term aggressions.