In-vivo Activity of IFN-λ and IFN-α Against Bovine-Viral-Diarrhea Virus in a Mouse Model

• DOI: 10.3389/fvets.2020.00045
• Publication Date: 2020-02-05T14:18:35Z
• Authors: María Eugenia Qui...; Lucas José Barone; Myrian Vanesa Trotta; Cecilia Turco; Florencia Celeste...; Alejandra Victori...; Nancy Patricia Ca...
• Keywords: Viremia; Infectivity

Bovine-viral-diarrhea virus (BVDV) can cause significant economic losses in livestock. The disease is controlled with vaccination and bovines are susceptible until vaccine immunity develops may remain vulnerable if a persistently infected animal left on the farm; therefore, an antiviral agent that reduces infectivity be useful tool control programs. Although many compounds promising in-vitro efficacy have been identified, lack of laboratory-animal models limited their potential for further clinical development. Recently, we described activity type I III interferons, IFN-α IFN-λ respectively, against several BVDV strains in-vitro. In this study, analyzed in-vivo both IFNs using BALB/c-mouse model. Mice two type-2 field developed viremia different kinetics, depending infecting strain's virulence, persisted 56 days post-infection (dpi). low-virulence strain elicited high systemic TNF-α levels at 2 dpi. were first applied subcutaneously one day before or after infection. reduced whether either was second experiment, increased number applications IFNs. All treatments compared to untreated mice. application pre- over time. This study proof concept potency in-vivo, thus encouraging trails use cytokine cattle.