Tamoxifen triggers the in vitro release of neutrophil extracellular traps in healthy horses

• DOI: 10.3389/fvets.2022.1025249
• Publication Date: 2023-01-06T19:33:27Z
• Authors: Constanza Salinas; Kassandra Barriga; Alejandro Albornoz; Pablo Alarcon; John Quiroga; Benjamín Uberti; José Sarmiento; Claudio Henriquez; Pamela Ehrenfeld; Rafael A. Burgos; Gabriel Moran
• Keywords: Neutrophil Extracellular Traps

Neutrophils display an array of biological functions including the formation neutrophil extracellular traps (NETs), web-like structures specialized in trapping, neutralizing, killing and preventing microbial dissemination within host. However, NETs contribute to a number inflammatory pathologies, severe equine asthma. Tamoxifen (TX) is selective estrogen receptor modulator which belongs triphenylethyllenes group molecules, used as treatment all stages estrogen-positive human breast cancer. Our previous results suggest that tamoxifen can modulate functionality promote resolution inflammation; this would partly explain clinical beneficial effect drug horses with airway inflammation. Enhanced production has been reported use humans, but minimal data exists regarding drug's on horses. The aim study assess vitro TX from peripheral blood healthy Five clinically mixed-breed adult were enrolled study. For this, cellular free DNA quantification, immunofluorescence for visualization NETs, assessment different types detection mitochondrial superoxide. induced at concentration 10 uM. show only two by TX: 95% spread ( sprNETs ) 5% aggregated aggNETs ). Furthermore, induction these could be influenced ROS. Future research should involve In vivo asthma treatment, evaluate BALF NET formation. conclusion, suggests inflammation due inhibition other neutrophilic not