Use of Citrated Whole Blood for Point-of-Care Viscoelastic Coagulation Testing in Dogs

• DOI: 10.3389/fvets.2022.827350
• Publication Date: 2022-03-07T09:10:23Z
• Authors: Whitney York; M. Ryan Smith; Chin-Chi Liu
• Keywords: Vacutainer; Cartridge; Venipuncture; Coagulation testing; Beagle; Phlebotomy; Thromboelastometry

A new, portable bedside coagulation monitor (VCM Vet) has provided a user-friendly, cartridge-based method to perform viscoelastic testing. However, the use of native whole blood limits time analyze sample minutes. The objective this study is assess whether citrated can be utilized with system and results are comparable those blood. secondary viability after up 4 hours resting.The population consisted 10 healthy mixed breed dogs. Whole samples were collected via jugular venipuncture. Blood was immediately transferred VCM test cartridge for control group analysis per manufacturer's instructions, remainder used fill two 3.2% sodium citrate vacutainer tubes. Test performed on from each tube concurrently rest period 30 min (baseline), 2 h, h. Citrated recalcified prior introduction into cartridge. Data recorded all reported parameters. Results groups compared baseline differences. Overall using ANOVA models. Where found, specific differences evaluated Tukey's test. Within-sample variation investigated as median (range). p < 0.05 considered significant.Samples obtained total runs 20 runs. Comparison controls revealed significant in CT (p 0.001) MCF 0.002). There no between baselines any parameter. Selected coefficients 6.8% (0-68.8%) CT, 2.4% (0-19.46%) alpha angle, (0-27.4%) MCF, 0% (0-16.3%) 45-min LY45.Citrated Vet device; however, new reference intervals will required. not significantly different h resting.