Immunopathological comparison of in ovo and post-hatch vaccination techniques for infectious bursal disease vaccine in layer chicks

• DOI: 10.3389/fvets.2022.947522
• Publication Date: 2022-07-26T09:20:43Z
• Authors: Iqra Zaheer; Wei Chen; Ahrar Khan; Abdelmotaleb Elokil; Muhammad Kashif S...; Tean Zaheer; Muhammad Zargham ...
• Keywords: In ovo; Infectious bursal disease; Embryonated; Antibody titer; Newcastle Disease

This study was designed to compare immunopathological effects of in ovo vaccination with post-hatch against IBD White Leghorn chicks. A total 189 embryonated eggs were divided into six groups. At day 18 incubation, groups A-C administered 228E, Winterfield 2512:10/3 and 2512/90:10/2.7, respectively, group D (post-hatch vaccination) E as shamed control (for quality evaluation technique), F control. The results showed that antibody titers detected by ELISA on days 2, 17, 28 significantly higher all compared F. On vaccinated (in vaccinated) no significant differences among themselves; however, at 28, only the followed cell-mediated immunity determined PHA-P assay than non-vaccinated No clinical signs infection observed any There increase bursa size intermediate plus strains (groups A, C, D) 28. histopathology treatment had mild lesions induced virus bursa. concluded live vaccines provides protective chickens even presence IBD-specific MDA; therefore, onset much earlier also maintained for longer time.