Porcine circovirus type 3: immunohistochemical detection in lesions of naturally affected piglets

• DOI: 10.3389/fvets.2023.1174718
• Publication Date: 2023-05-04T04:23:06Z
• Authors: Franciéli Adriane...; Bruno Albuquerque...; Bianca Santana de...; Caroline Pissetti; Lauren Ventura; Luciano Brandalise; Gustavo Simão; Fabio Vanucci; Tatiane Terumi Ne...; Itabajara da Silv...; David Driemeier
• Keywords: Mesenteric lymph nodes; Immunolabeling

This study aimed to evaluate the relationship between porcine circovirus type 3 (PCV3) viral load and histopathological findings in perinatal piglet tissues develop an immunohistochemical method for detecting virus lesions. The quantitative polymerase chain reaction (qPCR) cycle threshold (Ct) when amplifying PCV3 DNA area of perivascular inflammatory infiltrates different organs [central nervous system (CNS), lung, heart, liver, spleen, lymph nodes] were compared. To immunohistochemistry technique, rabbit sera produced against PCV3-capsid protein peptides selected using bioinformatic analyses. assay was initially implemented a tissue sample previously tested qPCR situ hybridization optimize procedure reagent dilutions. performance, samples from another 17 cases analyzed standardized parameters. most common microscopic lesion multisystemic periarteritis, with associated vasculitis, as mesenteric vascular plexus is one affected organs. Other tissues, such CNS, skeletal muscle, also affected. Comparison Ct values showed no significant difference, except lymphoid (spleen nodes), which had significantly higher loads than CNS tissues. There correlation infiltrates. revealed granular immunolabeling, mainly cytoplasm cells plexus, kidney, spleen.