Evaluation of the protective efficacy of six major immunogenic proteins of Mycoplasma Synoviae

• DOI: 10.3389/fvets.2023.1334638
• Publication Date: 2024-01-04T04:58:13Z
• Authors: Shuizhong Han; Ying Wang; Wenchi Chang; Lizhen Wang; Junyang Fang; Jingjing Han; Xiaolan Hou; Xuefeng Qi; Jingyu Wang
• Keywords: Mycoplasma synoviae

Mycoplasma synoviae ( MS ) is a primary avian pathogen prevalent worldwide that causes airsacculitis and synovitis in birds. Vaccination recommended as the most cost-effective strategy control of infection. Novel alternative vaccines are needed for eradicating controlling infection flocks. DnaK, enolase, elongation factor Tu (EF-Tu), MSPB, NADH oxidase LP78 major immunogenic antigens promising targets subunit vaccine candidates. In present study, genes encoding EF-Tu, LP78, were cloned expressed Escherichia coli . Enzyme-linked immunosorbent assay showed six recombinant proteins recognized by convalescent sera, indicating they during Two injections induced robust antibody response increased concentrations IFN-γ IL-4, especially rEnolase rEF-Tu. The proliferation peripheral blood lymphocytes was enhanced all immunized groups. Chickens with rEnolase, rEF-Tu, rLP78, rMSPB conferred significant protection against infection, indicated significantly lower DNA copies trachea, scores air sac lesions, lesser tracheal mucosal thickness than challenge control. Especially, provided best protective efficacy, followed rMSPB, rLP78. Our finds demonstrate bacterin can only reduce lesions caused but not prevent colonization organism. findings may contribute to development novel agents