Comparing different sperm concentrations for optimizing cooled semen use in the dog

• DOI: 10.3389/fvets.2023.1339840
• Publication Date: 2024-01-29T14:35:40Z
• Authors: Nicole Sugai; Stephen Werre; Julie T. Cecere; Orsolya Balogh
• Keywords: Semen quality; Semen extender; Semen Analysis

The use of shipping canine semen for artificial insemination has bloomed over the last 20 years. This allows spread genetic material while overcoming geographical or time-related challenges. optimal sperm concentration cooled transport in dog is unknown. Often extended 1:3–5 vol:vol without standardized concentrations shipment. We compared different storage and hypothesized that lower would result better quality. Semen was collected from healthy client-owned dogs ( n = 8). Individual ejaculates were divided into a control aliquot (CON) 1:3 with commercial extender. remaining sample centrifuged to 200 ×10 6 sperm/ml (C200), then serially diluted 100, 50, 25 (C100-C25). Aliquots 24 h re-extended. Sperm concentration, plasma membrane integrity (PMI, %), motility (subjective total, STM; computer-assisted analysis (CASA) total progressive, TM, PM; normal morphology (NM, %) assessed raw (T0), post-extension (T1), after cooling (T2), processing at (T3). Cooling resulted significant declines STM NM all groups decreased PMI CON C25-50. After (at T2), significantly C25 higher C25-100 p ≤ 0.038). Processing re-extension further spermiogram parameters. At T3, similar C200 but than C25-100, had lowest PMI. For parameters NM, performed worse most other groups. Comparing T3 C25-200 T2, PMI, STM, C25-200, C200, C100-200, respectively. In conclusion, our results show final extending centrifugation preferred based on highest If volume restrictions apply, desired collection facility superior adjustment storage.