Safety assessment of equine allogeneic tenogenic primed mesenchymal stem cells in horses with naturally occurring tendon and ligament injuries

• DOI: 10.3389/fvets.2024.1282697
• Publication Date: 2024-02-26T04:51:25Z
• Authors: Stephanie Carlier; Eva Depuydt; Lore Van Hecke; Ann Martens; Jimmy Saunders; Jan H. Spaas
• Keywords: 0403 veterinary science; desmitis; primed MSCs; Veterinary medicine; SF600-1100; tendonitis; Veterinary Science; 04 agricultural and veterinary sciences; mesenchymal stem cell; equine

Background Mesenchymal stem cells provide a valuable treatment option in orthopedic injuries horses. Objectives The aim of this study was to evaluate the hematological, biochemical, immunological and immunomodulatory parameters following intralesional with tenogenic primed equine allogeneic peripheral blood-derived mesenchymal (tpMSCs) client-owned horses naturally occurring superficial digital flexor tendon (SDFT) suspensory ligament (SL) injuries. Methods immunogenicity capacities tpMSCs were assessed modified mixed lymphocyte reaction, including blood mononuclear (PBMCs) 14 SDFT SL after tpMSCs. In second study, 18 received either an injection ( n = 9) or no 9). Results did not provoke cellular immune response p < 0.001) able immunomodulate stimulated T lymphocytes vitro . Therapeutic use result relevant hematologic biochemical abnormalities. Main limitations Both studies had small sample size. No statistical analyses performed study. Fibrinogen only analyzed single horse prior treatment. Conclusion Co-incubation PBMCs that have been previously exposed elicit lymphocytes. Intralesional abnormal changes hematological parameters.