In vitro and in vivo anthelmintic efficacy of condensed tannins extracted from the seeds of alfalfa (Medicago sativa L.) against Haemonchus contortus infection

• DOI: 10.33899/ijvs.2022.133537.2247
• Publication Date: 2022-12-28T06:23:14Z
• Authors: Omnia Kandil; Hatem A. Shalaby; Seham H. Hendawy; Mohamed S. Abdelf...; Doaa Sedky; Noha M. Hassan; Amira H. El Namaky; Heba M. Ashry; Nadia M. Abu EL Ezz; Mona S. Mahmoud; Abdulla A. Mahmoud
• Keywords: Albendazole; Condensed tannin

This study was designed to examine in vitro and vivo anthelmintic efficacy of condensed tannins (CT) extracted from seeds Medicago sativa on Haemonchus contortus sheep. CT's effect assessed at a 300 μg/ml concentration compared with albendazole (reference drug) 10 μg/ml. The results showed that CT had nematocidal H. contortus, the cuticle adult worm appeared be its initial target. For experiment, nine 3-month-old helminths-free lambs were distributed into three groups. Group 1 (n=3) challenged only as infected untreated controls; 2 treated tannin, 3 albendazole. Fecal blood samples collected every days until end experiment; for fecal egg count (FEC) anti- IgG titers determination, respectively. G2 exhibited pronounced decrease mean FEC great FECR% detected first-week post-treatment (PT) experiment G1 animals. antibody levels gradually increased following 2nd dose treatment other A brilliant consistent relation between elevation response reduction observed second booster dosing G2. In conclusion, evoked strongly activity against could used natural alternative high potency haemonchosis