22KD Zein Content Coordinates Transcriptional Activity during Starch Synthesis in Maize Endosperm

• DOI: 10.3390/agronomy10050624
• Publication Date: 2020-04-28T13:57:21Z
• Authors: Ada Menie Nelly S...; Hailiang Zhao; Yao Qin; Qin Sun; Dianming Gong; Zhenyuan Pan; Fazhan Qiu
• Keywords: Transcription

Starch, the main form of stored energy in plants, plays an important role maize (Zea mays L.) kernel development. The Shrunken-2 (Sh2) gene encodes large subunit rate-limiting starch biosynthetic enzyme ADP-glucose pyrophosphorylase (AGPase). sh2 mutant exhibits impaired AGPase activity, resulting partial or complete loss synthesis. Here, we investigated transcriptional regulatory framework through transcriptome and co-expression network analysis using F2 population derived from reference line B73 sweet corn inbred HZ508. We identified 5175 differentially expressed genes (DEGs), including 2878 upregulated 2297 downregulated lines. DEGs are associated with various biological processes nutrient reservoir transferase catalytic water deprivation glycogen metabolism. At genetic level, 2465 DEGs, 357 transcription factors, were involved transcription. In addition, floury opaque fl1, ndk2, o7 o2, which regulate biosynthesis 22KD zein, co-expressed differential factor genes, thus suggesting that zein content might be a key regulator coordinating expression determining accumulation endosperm.