Modulation and Protection Effects of Antioxidant Compounds against Oxidant Induced Developmental Toxicity in Zebrafish

• DOI: 10.3390/antiox9080721
• Publication Date: 2020-08-10T09:07:23Z
• Authors: Nuria Boix; Elisabet Teixido; Ester Pique; Juan Maria Llobet; Jesus Gomez-Catalan
• Keywords: Zebra danio; 0303 health sciences; Estrès oxidatiu; Peix zebra; in vivo model; antioxidant effect; RM1-950; Toxicology; Antioxidants; Article; 3. Good health; 03 medical and health sciences; Oxidative stress; oxidative stress; zebrafish embryo; Therapeutics. Pharmacology; Toxicologia

The antioxidant effect of compounds is regularly evaluated by in vitro assays that do not have the capability to predict vivo protective activity or determine their underlying mechanisms action. aim this study was develop an experimental system evaluate effects different compounds, based on zebrafish embryo test. Zebrafish embryos were exposed tert-butyl hydroperoxide (tBOOH), tetrachlorohydroquinone (TCHQ) and lipopolysaccharides from Escherichia coli (LPS), chemicals are known inducers oxidative stress zebrafish. developmental toxic (lethality dysmorphogenesis) induced these modulated with n-acetyl l-cysteine Nω-nitro l-arginine methyl ester hydrochloride, dimethyl maleate dl-buthionine sulfoximine order validate oxidant mechanism inducers. tBOOH, TCHQ, LPS confirmed determination significant differences comparison between concentration-response curves modulators status. This concept also applied well-known antioxidants, such as vitamin E, quercetin, lipoic acid. Our results confirm model useful tool test compounds.