Identification of Previously Unrecorded Bacills, Serratia, and Mucor Strains Isolated from Yogurt

• DOI: 10.3390/app132312819
• Publication Date: 2023-11-29T14:00:18Z
• Authors: Jehan S. Al-brahim; Omer A. Abdalla; Alanoud I. Alwarqan
• Keywords: Mucor circinelloides; Serratia; Cereus; Bacillus (shape)

(1) Background: Foodborne illnesses are recognized as a significant threat to public health and the economy in both developed developing nations. The safety of foods containing microorganisms has consequently become major worldwide concern. One most frequent causes food deterioration world is microbial contamination. (2) Methods: Yogurt containers that were bought commercially Riyadh, Saudi Arabia during their validity period contained four different species bacteria one type fungus. Using nutritional agar Czapek-Dox medium, fungi isolated. isolates identified using scanning microscope. further classified for molecular evolutionary analyses 16S rRNA ITS sequences from fungi, respectively, conjunction with universal primers 27F, 1492R, ITS1F, ITS4R. (3) Results: A total 131 separate strains 12 yogurt samples based on phenotypic characteristics. In total, 79 (60.3%) consisted Serratia marcescens, Bacillus subtilis, Mucor circinelloides, 52 (39.7%) being cereus. While cells shaped like rods, sporangia large, round, black. Each strain was by its accession number, which follows: MK590996.1: B. cereus; MK591144.1: subtilis; MK591002.1 MK591014.1: S. marcescens; MK559692.1: M. circinelloides. maximum identification found be between 98.64 100% when BLAST used compare NCBI GeneBank database. (4) Conclusions: Genus performed similarity score values. products high concentrations cereus carry risk hazards due potential spreading pathogenic humans.