Inflammatory Stress Induced by Intraperitoneal Injection of LPS Increases Phoenixin Expression and Activity in Distinct Rat Brain Nuclei

• DOI: 10.3390/brainsci12020135
• Publication Date: 2022-01-21T03:40:20Z
• Authors: Tiemo Friedrich; Martha Anna Schalla; Miriam Goebel-Ste...; Peter Kobelt; Matthias Rose; Andreas Stengel
• Keywords: Solitary tract; Dorsal motor nucleus; Supraoptic nucleus; Dorsal raphe nucleus; Area postrema; Raphe nuclei; Corticosterone

Due to phoenixin's role in restraint stress and glucocorticoid stress, as well its recently shown effects on the inflammasome, we aimed investigate of lipopolysaccharide (LPS)-induced inflammatory activity brain nuclei-expressing phoenixin. Male Sprague Dawley rats (n = 6/group) were intraperitoneally injected with either LPS or control (saline). Brains processed for c-Fos phoenixin immunohistochemistry resulting slides evaluated using ImageJ software. was counted densitometry. significantly increased expression central amygdaloid nucleus (CeM, 7.2-fold), supraoptic (SON, 34.8 ± 17.3 vs. 0.0 0.0), arcuate (Arc, 4.9-fold), raphe pallidus (RPa, 5.1-fold), bed stria terminalis (BSt, 5.9-fold), dorsal motor vagus nerve (DMN, 89-fold), medial part solitary tract (mNTS, 121-fold) compared control-injected group (p < 0.05). Phoenixin also CeM (1.2-fold), SON (1.5-fold), RPa (1.3-fold), DMN mNTS (1.9-fold, p 0.05), leading a positive correlation between RPa, BSt, In conclusion, induces significant increase immunoreactive nuclei that is distinctively different from stress.