The Genes—Candidates for Prognostic Markers of Metastasis by Expression Level in Clear Cell Renal Cell Cancer

• DOI: 10.3390/diagnostics10010030
• Publication Date: 2020-01-09T08:07:11Z
• Authors: Natalya Apanovich; Maria Peters; Pavel Apanovich; Danzan Mansorunov; Anna Markova; Vsevolod Matveev; Alexander Karpukhin
• Keywords: Medicine (General); 03 medical and health sciences; R5-920; 0302 clinical medicine; renal cancer; gene expression; markers; Article; 3. Good health

The molecular prognostic markers of metastasis are important for personalized approaches to clear cell renal carcinoma (ccRCC) treatment but practical use still missing. To address this gap we studied the expression ten genes—CA9, NDUFA4L2, VWF, IGFBP3, BHLHE41, EGLN3, SAA1, CSF1R, C1QA, and FN1—through RT-PCR, in 56 ccRCC patients without metastases with metastases. All these, excluding showed differential increased (besides SAA1) non-metastasis tumors. gene levels tumors were decreased, besides FN1 (not changed), SAA1 (increased). There significant associations differentially expressed genes by ROC analysis Fisher exact test. association C1QA is shown first time. CA9, BHLHE4, EGLN3 distinguished as strongest candidates biomarkers. We used an approach that presupposed marker was any three from selected panel received sensitivity (88%) specificity (73%) a relative risk RR > 3. In conclusion, genes—the biomarkers metastasis—was created results might shed some light on processes.