MicroRNA Profile of Patients with Chronic Limb-Threatening Ischemia

• DOI: 10.3390/diagnostics10040230
• Publication Date: 2020-04-21T07:23:06Z
• Authors: Muzammil H. Syed; Abdelrahman Zamzam; Jason Valencia; Hamzah Khan; Shubha Jain; Krishna K. Singh; Rawand Abdin; Mohammad Qadura
• Keywords: Fold change

Chronic limb-threatening ischemia (CLTI) results in devastating complications such as lower-limb amputations. In this study, a genome-wide plasma microRNAs (miRNA) sequencing was performed to identify miRNA(s) associated with CLTI. Blood samples were collected from early stage CLTI patients (ABI < 0.9) and non-PAD controls ≥ for 3 experiments: discovery phase (n = 23), confirmatory 52) validation 20). the phase, next generation (NGS) used miRNA circulating 13) patients, compared 10). Two down-regulated miRNAs (miRNA-6843-3p miRNA-6766-5p) three upregulated (miRNA-1827, miRNA-320 miRNA-98-3p) identified (≥2-fold change). these 5 deregulated further investigated 21) CTLI 31) using qRT-PCR. Only miRNA-1827 found be significantly (≥3-fold, p-value 0. 001) group. Lastly, minimize influence of confounding factors, levels validated third cohort 10) matched Our analysis demonstrated that expression increased (≥2-folds, 0.001). summary, is elevated