Bioethanol: A New Synergy between Marine Chitinases from Bacillus haynesii and Ethanol Production by Mucor circinelloides

• DOI: 10.3390/fermentation9010040
• Publication Date: 2023-01-02T08:50:32Z
• Authors: Vishnupriya Govin...; Arun Kumar Subramani; Ramya Gopalakrishnan; Se-Kwon Kim; Ritu Raval; Keyur Raval
• Keywords: Mucor circinelloides; Chitinase; Biopolymer

The fourth generation of bioethanol production is on a lookout for non-lignocellulosic biomass waste. One such candidate chitin, the second most abundant biopolymer earth. However, crystalline nature chitin hinders its application potential production. This limitation can be circumvented by hydrolysing this polymer into oligomers using chitinases. We used hypothesis and isolated Bacillus haynesii, marine bacterium that utilizes colloidal as substrate produces oligosaccharides. Further, we utilized Mucor circinelloides to produce oligosaccharides in shake flask. investigated effect inoculum age, filling volume, different substrates, concentration from haynesii-produced haynesii demonstrated maximum chitinase activity 3.08 U/mL with specific 96 U/mg at 90th h. Chitin produced were confirmed mass spectrometry. Bioethanol was determined dichromate oxidation assay well gas chromatography. research resulted 7.4 g/L ethanol 30 oligosaccharides, yield 0.25 g ethanol/g 55th h 48 80 mL fermentation medium. Results suggest are an effective renewable