Optimization, Scale-Up, and Economic Analysis of the Ethanol Production Process Using Sargassum horneri

• DOI: 10.3390/fermentation9121004
• Publication Date: 2023-11-29T14:00:18Z
• Authors: InYung Sunwoo; Yoojin Kim; Jieun Kim; HyunJin Cho; Gwi-Taek Jeong
• Keywords: Enzymatic Hydrolysis; Ethanol Fermentation

Recently, the extensive spread of some algae along coastlines has surged into unmanageable thick decomposition layers. This study aimed to demonstrate use Sargassum horneri as a biomass resource for ethanol production through continuous hydrolysis, enzymatic saccharification, and fermentation process. Sugars from S. were obtained using combination thermal acid hydrolysis saccharification. The optimal conditions involved 10% (w/v) slurry treated with 100 mM H2SO4 at 121 °C 60 min; saccharification 16 U/mL Cellic CTec2 further boosted monosaccharide concentration 23.53 g/L. Fermentation experiments conducted mannitol-adapted Saccharomyces cerevisiae BY4741 hydrolysate. Enhanced was observed in hydrolysate, particularly BY4741, which yielded 10.06 g/L ethanol. Non-adapted produced 8.12 ethanol, it primarily utilized glucose not mannitol. Regarding 5 L- 500 L-scale fermenters, concentrations reached 10.56 7.88 yields 0.51 0.45, respectively, 48 h. confirmed economic viability waste seaweed optimized pretreatment adaptive evolution