A Low Molecular Weight Hyaluronic Acid Derivative Accelerates Excisional Wound Healing by Modulating Pro-Inflammation, Promoting Epithelialization and Neovascularization, and Remodeling Collagen

• DOI: 10.3390/ijms20153722
• Publication Date: 2019-07-30T15:15:56Z
• Authors: Yin Gao; Yao Sun; Hao Yang; Pengyu Qiu; Zhongcheng Cong; Yifang Zou; Liu Song; Jianfeng Guo; Tassos P. Anastas...
• Keywords: Male; 0303 health sciences; <i>N</i>-butyrylation; Macrophages; Anti-Inflammatory Agents; Neovascularization, Physiologic; anti-inflammation; Article; Rats; 3. Good health; hyaluronan; lymphangiogenesis; angiogenesis; 03 medical and health sciences; Re-Epithelialization; Human Umbilical Vein Endothelial Cells; Animals; Humans; Collagen; Hyaluronic Acid; Rats, Wistar

Recent knowledge of the cellular and molecular mechanisms underlying cutaneous wound healing has advanced development medical products. However, patients still suffer from failure current treatments, due to complexity process thus novel therapeutic approaches are urgently needed. Previously, our laboratories produced a range low weight hyaluronic acid (LMW-HA) fragments, where proportion glucosamine moieties were chemically N-acyl substituted. Specifically, N-butyrylation results in anti-inflammatory properties macrophage system, we demonstrate importance substituents modulating inflammatory response LMW-HA. We have set up an inter-institutional collaborative program examine biomedical applications N-butyrylated LMW-HA (BHA). In this study, potentials BHA for dermal assessed vitro vivo. Consequently, significantly promotes relative commercial care product. By contrast, "parent" partially de-acetylated (DHA) re-acetylated DHA (AHA) delays closure, demonstrating specificity N-acylation healing. Mechanistic studies reveal that BHA-mediated effect is achieved by targeting three phases (i.e., inflammation, proliferation maturation), significant potential clinical translation