Single-Cell Transcriptomic Profiling in Inherited Retinal Degeneration Reveals Distinct Metabolic Pathways in Rod and Cone Photoreceptors

• DOI: 10.3390/ijms232012170
• Publication Date: 2022-10-13T00:53:29Z
• Authors: Yiyi Chen; Yujie Dong; Jie Yan; Lan Wang; Shirley Yu; Kangwei Jiao; François Paquet-D...
• Keywords: Photoreceptor cell; Reprogramming; Cell type

The cellular mechanisms underlying hereditary photoreceptor degeneration are still poorly understood. aim of this study was to systematically map the transcriptional changes that occur in degenerating mouse retina at single cell level. To end, we employed single-cell RNA-sequencing (scRNA-seq) and retinal degeneration-1 (rd1) mice profile impact disease mutation on diverse types during early post-natal development. transcriptome data allowed annotate 43,979 individual cells grouped into 20 distinct clusters. We further characterized cluster-specific metabolic biological types. Our results highlight Ca2+-signaling as relevant degeneration. Although reprogramming retina, known 'Warburg effect', has been documented, were noticed rd1 mice. Such likely regulated through mitogen-activated protein kinase (MAPK) pathway. By combining transcriptomes immunofluorescence staining, our revealed type-specific gene expression, well interplay between Ca2+-induced death pathways.