Tissue Response to a Porous Collagen Matrix Used for Soft Tissue Augmentation

• DOI: 10.3390/ma12223721
• Publication Date: 2019-11-12T09:07:07Z
• Authors: Jordi Caballé-Ser...; Sophia Zhang; Luca Ferrantino; Massimo Simion; Vivianne Chappuis; Dieter D. Bosshardt
• Keywords: Matrix (chemical analysis); Biomaterial; Dense connective tissue

A short inflammatory phase and fast ingrowth of blood vessels mesenchymal cells are essential for tissue integration a biomaterial. Macrophages play key role in this process. We investigated invasion macrophages, vessels, proliferating into highly porous volume-stable collagen matrix (VCMX) used soft augmentation around teeth dental implants. The biomaterial was implanted submucosal pouches the canine maxilla, response analyzed at six different time points. Immunohistochemistry done (PCNA), macrophages (MAC387), multinucleated giant (CD86), (TGM2). Blood rapidly filled VCMX pores. During first week, MAC387+ populated pores, PCNA+ invaded VCMX, CD86+ scattered were observed. At 15 days, scanty, had completely number peaked, fibroblasts appeared. 30 absent, numbers declined, while vessel fibroblast high. 90 residual well-integrated connective tissue. In conclusion, elicited followed by rapid integration.