Parallel Evolution to Elucidate the Contributions of PA0625 and parE to Ciprofloxacin Sensitivity in Pseudomonas aeruginosa

• DOI: 10.3390/microorganisms11010013
• Publication Date: 2022-12-21T08:59:26Z
• Authors: Qi Liu; Liwen Yin; Chenjing Lv; Fang Bai; Zhihui Cheng; Weihui Wu; Yongxin Jin
• Keywords: <i>Pseudomonas aeruginosa</i>; 0301 basic medicine; 03 medical and health sciences; QH301-705.5; Communication; PA0625; pyocin; <i>ParE</i>; ciprofloxacin resistance; Biology (General); 3. Good health

Pseudomonas aeruginosa is a ubiquitous pathogen that causes wide range of acute and chronic infections. Ciprofloxacin, one the first-line fluoroquinolone class antibiotics, commonly used for treatment P. However, ciprofloxacin-resistant increasingly reported worldwide, making difficult. To determine resistance-related mutations, we conducted an experimental evolution using previously identified clinical isolate, CRP42. The evolved mutants could tolerate 512-fold higher concentration ciprofloxacin than Genomic DNA reference mapping was performed, which revealed mutations in genes known to be associated with resistance as well those not linked resistance, including ParER586W substitution PA0625 frameshift insertion. Simulation insertion by gene editing CRP42 model strain PAO1 demonstrated while mutation does contribute but rather affects tolerance against ciprofloxacin. These findings advance our understanding aeruginosa.