The Pathogenicity of Fusobacterium nucleatum Modulated by Dietary Fibers—A Possible Missing Link between the Dietary Composition and the Risk of Colorectal Cancer

• DOI: 10.3390/microorganisms11082004
• Publication Date: 2023-08-03T15:13:06Z
• Authors: Sadia Nawab; Qelger Bao; Lin-Hua Ji; Qian Luo; Xiang Fu; Shuxuan Fan; Zixin Deng; Wei Ma
• Keywords: Fusobacterium nucleatum; Proinflammatory cytokine

The dietary composition has been approved to be strongly associated with the risk of colorectal cancer (CRC), one most serious malignancies worldwide, through regulating gut microbiota structure, thereby influencing homeostasis colonic epithelial cells by producing carcinogens, i.e., ammonia or antitumor metabolites, like butyrate. Though butyrate-producing Fusobacterium nucleatum considered a potential tumor driver chemotherapy resistance and poor prognosis in CRC, it was more frequently identified healthy individuals rather than CRC tissues. First, within concentration range tested, fermentation broth F. exhibited no significant effects on Caco-2 NCM460 viability except for notable up-regulation expression TLR4 (30.70%, p < 0.0001) Myc (47.67%, = 0.021) genes encoding proinflammatory cytokines including IL1B (197.57%, 0.0001), IL6 (1704.51%, IL8 (897.05%, exclusively. Although marked polydextrose fibersol-2 growth nucleatum, were observed, once culture media supplemented fibersol-2, corresponding broths significantly inhibited up 48.90% (p 0.0003, 72 h, 10%) 52.96% 0.0002, 10%), respectively dose-dependent manner. These two kinds fibers considerably promoted butyrate production 205.67% 0.0001, 6% at 24 h) 153.46% 12 h), which explained why how cultured suppressing cells. Above findings indicated that fiber determined carcinogenic bacterium, played an important role association between composition, primarily content fibers, CRC.