A bvrR/bvrS Non-Polar Brucella abortus Mutant Confirms the Role of the Two-Component System BvrR/BvrS in Virulence and Membrane Integrity

• DOI: 10.3390/microorganisms11082014
• Publication Date: 2023-08-05T14:21:39Z
• Authors: Olga Rivas-Solano; Kattia Núñez-Montero; Pamela Altamirano...; Nazareth Ruiz-Vil...; Elías Barquero-Calvo; Edgardo Moreno; Esteban Chaves-Ol...; Caterina Guzmán-V...
• Keywords: Sanger sequencing; Southern blot

Brucella abortus is a bacterial pathogen causing bovine brucellosis worldwide. This facultative extracellular–intracellular can be transmitted to humans, leading zoonotic disease. The disease remains public health concern, particularly in regions where livestock farming present. two-component regulatory system BvrR/BvrS was described by isolating the attenuated transposition mutants bvrR::Tn5 and bvrS::Tn5, whose characterization led understanding of role survival. However, phenotypic comparison with deletion has not been performed because their construction unsuccessful brucellae difficult phylogenetically related Rhizobiales orthologs. Here, we used an unmarked gene excision strategy generate B. mutant strain lacking both genes, called ∆bvrRS. verified through PCR, Southern blot, Western Sanger sequencing, whole-genome confirming clean mutation without further alterations at genome level. ∆bvrRS shared traits mutants, pathogenesis membrane integrity. non-antimicrobial marker excellent tool for continuing studies on lifestyle.