Biocontrol Effect of Bacillus velezensis D7-8 on Potato Common Scab and Its Complete Genome Sequence Analysis

• DOI: 10.3390/microorganisms13040770
• Publication Date: 2025-03-28T10:45:51Z
• Authors: Yu Jiang; Pengfei He; Huihui Kong; Pengbo He; Yixin Wu; Guowen Tang; Ping Tang; Yining Di; Xingyu Li; Lufeng Liu; Shahzad Munir; Yueqiu He

Potato common scab, caused by Streptomyces species, is a widespread soil-borne disease that poses a significant threat to potato cultivation globally. In this study, a Bacillus velezensis D7-8 strain was isolated from a potato. This endophytic bacterium exhibited broad-spectrum antifungal activity, and pot trials demonstrated that the D7-8 strain effectively controlled potato common scab with an efficacy of 42.07%. The complete genome sequence of the D7-8 strain was sequenced and subsequently identified as B. velezensis through multiple bioinformatic methods, primarily through structural variation analysis of whole-genome sequences. The machine learning method predicted that the expression profiles of colinear genes among closely related Bacillus species were highly consistent. Metabolite analysis of crude extracts using ultra-high-performance liquid chromatography coupled with quadrupole-Orbitrap high-resolution mass spectrometry (UPLC-Q-Exactive HRMS) revealed that D7-8 produces bioactive compounds, including surfactin and fengycin, both known for their antimicrobial properties. This study elucidates the antagonistic effect of B. velezensis D7-8 against Streptomyces acidiscabies and provides a valuable reference for future research on accurate microbial identification.