Probiotic and Triticale Silage Fermentation Potential of Pediococcus pentosaceus and Lactobacillus brevis and Their Impacts on Pathogenic Bacteria

• DOI: 10.3390/microorganisms7090318
• Publication Date: 2019-09-05T07:22:36Z
• Authors: Ilavenil Soundhar...; Dahye Kim; Palaniselvam Kupp...; Karanan Muthusamy; Hyun Jeong Lee; Ki Choon Choi
• Keywords: Triticale; Silage; Lactobacillus brevis; Pediococcus

The purpose of this study was to identify potent lactic acid bacteria that could have a great impact on triticale silage fermentation at different moisture levels and determine their anti-bacterial activity high probiotic potential. For purpose, Pediococcus pentosaceus (TC48) Lactobacillus brevis (TC50) were isolated from fermented silage. ability these isolates in powder studied by an ensiling method. TC48 had higher ferment increasing the content than TC50. Extracellular supernatant (ECS) TC50 exhibited strong antibacterial effects (inhibition zone diameters: 18–28 mm) against tested cattle pathogenic with minimum inhibitory/ bactericidal concentrations (MIC/MBC) values 5.0–10 mg/mL 10–20 mg/mL, respectively. showed activities E. coli, P. aeruoginosa, S. aureus faecalis through destruction membrane integrity as confirmed decreased viability, increased 260 nm absorbing material culture filtrate exposed ECS both strains. strains tolerance artificial gastric, duodenal intestinal fluids. good hydrophobicity auto-aggregations properties. significantly co-aggregated time-dependent manner. In summary, all positive least one functional property during process. However, addition L. yielded greatest quality improvement, having