Emergence of a Multidrug-Resistant Enterobacter hormaechei Clinical Isolate from Egypt Co-Harboring mcr-9 and blaVIM-4

• DOI: 10.3390/microorganisms8040595
• Publication Date: 2020-04-21T07:23:06Z
• Authors: Ahmed M. Soliman; Fumito Maruyama; Hoda O. Zarad; Atsushi Ota; Hirofumi Nariya; Toshi Shimamoto; Tadashi Shimamoto
• Keywords: Colistin; MCR-1; Enterobacter cloacae; Enterobacteriaceae Infections

Abstract: This study describes the first full genomic sequence of an mcr-9 and blaVIM-4-carrying multidrug-resistant Enterobacter hormaechei clinical isolate from Egypt. The strain was isolated in April 2015 sputum a patient Cairo, blaVIM-4 genes were identified by PCR screening DNA sequencing; subjected to antimicrobial susceptibility testing, conjugation experiments, whole sequencing. carried IncHI2 plasmid, pAMS-38a (281,121 bp size); plasmid also conferring resistance against sulfonamides (sul1), quinolones (qnrA1), trimethoprim (dfrA1), β-lactams (blaTEM-1B), aminoglycosides (aac (6')-II, aadA23, aadA2b, ant(2'')-Ia). susceptible colistin (MIC, <0.25 μg/mL); this could be due absence qseC/qseB regulatory system located downstream Enterobacterales, which is involved induction colistin-resistance. genetic context as IS1-mcr-9-IS903-pcoS-∆pcoE-rcnA intI1-blaVIM-4-aac (6')-II-dfrA1-∆aadA23-smr-ISPa21-qacE∆1, respectively. report /IncHI2-carrying E. Africa Middle East. Plasmids group two insertion sequences (IS1, IS903) might main vehicles for dissemination mcr-9. Further essential identifying its incidence prevent dissemination.