Genomic Insight of VIM-harboring IncA Plasmid from a Clinical ST69 Escherichia coli Strain in Italy

• DOI: 10.3390/microorganisms8081232
• Publication Date: 2020-08-13T06:58:02Z
• Authors: Vittoria Mattioni...; Ibrahim Bitar; Aurora Piazza; Alessandra Mercato; Elena Fogato; Jaroslav Hrabak; Roberta Migliavacca
• Keywords: 0301 basic medicine; 570; QH301-705.5; Communication; E. coli; 610; <i>E. coli</i>; 3. Good health; 03 medical and health sciences; <i>IncA</i>; IncA; blaVIM-1; Biology (General); <i>bla</i>VIM-1

Background: VIM (Verona Integron-encoded Metallo-beta-lactamase) is a member of the Metallo-Beta-Lactamases (MBLs), and able to hydrolyze all beta-lactams antibiotics, except for monobactams, including carbapenems. Here we characterize VIM-producing IncA plasmid isolated from clinical ST69 Escherichia coli strain an Italian Long-Term Care Facility (LTCF) inpatient. Methods: An antimicrobial susceptibility test conjugation assay were carried out, transferability blaVIM-type gene was confirmed in transconjugant. Whole-genome sequencing (WGS) 550 performed using Sequel I platform. Genome assembly "Microbial Assembly". Genomic analysis conducted by uploading contigs ResFinder PlasmidFinder databases. Results: Assembly resulted three complete circular contigs: chromosome (4,962,700 bp), (p550_IncA_VIM_1; 162,608 harboring genes coding aminoglycoside resistance (aac(6')-Ib4, ant(3″)-Ia, aph(3″)-Ib, aph(3')-XV, aph(6)-Id), beta-lactam (blaSHV-12, blaVIM-1), macrolides (mph(A)), phenicol (catB2), quinolones (qnrS1), sulphonamide (sul1, sul2), trimethoprim (dfrA14), IncK/Z (p550_IncB_O_K_Z; 100,306 free antibiotic genes. Conclusions: The increase reports plasmids bearing different highlights overall important role disseminating carbapenemase genes, with preference blaVIM-1 Italy.