The Effect of a New N-hetero Cycle Derivative on Behavior and Inflammation against the Background of Ischemic Stroke

• DOI: 10.3390/molecules27175488
• Publication Date: 2022-08-29T07:34:56Z
• Authors: Denis A. Borozdenko; Tatiana A. Shmigol; Aiarpi A. Ezdoglian; Darya I. Gonchar; Natalia. Y. Karpe...; Dmitri N. Lyakhmun; Anastasia D. Shagina; Elvira A. Cherkas...; Daria D. Namestni...; Ilya L. Gubskiy; Anastasia A. Cher...; Nina M. Kiseleva; Vadim V. Negrebetsky; Yuri I. Baukov
• Keywords: Stroke

Ischemic stroke triggers a whole cascade of pathological changes in the brain, one which is postischemic inflammation. Since such cases thrombolytic therapy often not possible, methods that modulate inflammation and affect microglia become particularly interesting. We synthesized 3-(2-oxo-4-phenylpyrrolidin-1-yl)propane-1-sulfonate calcium(II) (Compound 4) studied its anti-inflammatory activity vitro vivo models ischemia. Macrophage cell line RAW 264.7 was treated with lipopolysaccharides (LPS) Compound 4 at various dosages to study cytokine profile using real-time PCR cytometric bead array (CBA). Stroke rats simulated by middle cerebral artery occlusion method (MCAO). Several tests were performed characterize neurological deficit locomotor rats, afterwards, postmortem, number astrocytes counted immunohistochemistry. dose-dependently reduced expression interleukin-1β (IL1β), inducible nitric oxide synthase (iNOS) genes culture increased concentration cytokines: interleukin-2, 4, 6 (IL-2, IL-4, IL-6). In orienting-exploratory behavior, motor deficit. The promote support lower group 4. volume measured magnetic resonance imaging (MRI) showed no difference. have shown demonstrates increasing synthesis reducing pro-inflammatory cytokines, positively affects rats. Thus, has high therapeutic potential management patients after requires further neuroprotective properties.