Covalent Immobilization of β-Glucosidase into Mesoporous Silica Nanoparticles from Anhydrous Acetone Enhances Its Catalytic Performance

• DOI: 10.3390/nano10010108
• Publication Date: 2020-01-06T08:48:48Z
• Authors: Filomena Sannino; Aniello Costantini; Francesco Ruffo; Antonio Aronne; Virginia Venezia; Valeria Califano
• Keywords: Anhydrous

An immobilization protocol of a model enzyme into silica nanoparticles was applied. This exploited the use bifunctional molecule triethoxysilylpropylisocyanate (TEPI) for covalent binding through linker suitable length. The β-glucosidase (BG) anchored onto wrinkled (WSNs). BG represents bottleneck in conversion lignocellulosic biomass biofuels cellulose hydrolysis and fermentation. key aspect procedure an organic solvent (anhydrous acetone) which not soluble. aimed to restrict its conformational changes thus preserve native structure. approach led biocatalyst with improved thermal stability, characterized by high efficiency yield. It found that apparent