Overcoming the Inflammatory Stage of Non-Healing Wounds: In Vitro Mechanism of Action of Negatively Charged Microspheres (NCMs)

• DOI: 10.3390/nano10061108
• Publication Date: 2020-06-04T08:36:09Z
• Authors: Edorta Santos-Viz...; Aiala Salvador; Claudia Vairo; Manoli Igartua; Rosa Maria Hernandez; Luis Correa; Silvia Villullas; Garazi Gainza
• Keywords: Viability assay

Negatively charged microspheres (NCMs) represent a new therapeutic approach for wound healing since recent clinical trials have shown NCM efficacy in the recovery of hard-to-heal wounds that tend to stay inflammatory phase, unlocking process. The aim this study was elucidate mechanism action. NCMs were extracted from commercial microsphere formulation (PolyHeal® Micro) and cytotoxicity, attachment, proliferation viability assays performed keratinocytes dermal fibroblasts, while macrophages used phagocytosis polarization assays. We demonstrated cells attach surface, are biocompatible promote cell at specific concentrations (50 10 NCM/cell) by minimum 3 fold compared control group. Furthermore, internalization seemed drive these noninflammatory condition, as over-expression CD206 under-expression CD64, M2 M1 markers, respectively. an effective reverting chronic state stagnant (such diabetic wounds) thus improving healing.