Anti-Atherogenic Effect of 10% Supplementation of Anchovy (Engraulis encrasicolus) Waste Protein Hydrolysates in ApoE-Deficient Mice
Fish Proteins
0301 basic medicine
Mice, Knockout, ApoE
Protein Hydrolysates
Anchovy by-products, ApoE-knockout mice, Atherosclerosis, Fish protein hydrolysates, Fish proteins
Article
Feces
Mice
03 medical and health sciences
Animals
ApoE-knockout mice
fish proteins
Hypolipidemic Agents
2. Zero hunger
Atherosclerosis
Lipid Metabolism
Plaque, Atherosclerotic
3. Good health
Mice, Inbred C57BL
Disease Models, Animal
Liver
Seafood
Dietary Supplements
fish protein hydrolysates
anchovy by-products
Female
Anti-Obesity Agents
atherosclerosis
DOI:
10.3390/nu13072137
Publication Date:
2021-06-23T02:10:59Z
AUTHORS (10)
ABSTRACT
Fish protein consumption exerts beneficial metabolic effects on human health, also correlating with a decreased risk for cardiovascular disease. Fish waste contains high amount of proteins and utilization may offer the opportunity for generating compounds advantageous for human health. Especially, fish waste protein hydrolysates beneficially influence pathways involved in body composition, exerting anti-inflammatory and antioxidant activities, making their potential supplementation in human disorders of increased interest. This study assessed the effect of a 10% (w/w) anchovy waste protein hydrolysate (APH) diet for 12 weeks in reducing atherosclerosis in ApoE−/− mice, through histological and immunohistochemical methods. In addition, monitoring of plaque development was performed, using high-frequency ultrasound and magnetic resonance imaging. Overall, the APH diet attenuated atherosclerotic plaque development, producing a regression of arterial lesions over time (p < 0.05). Twelve weeks on an APH diet had an anti-obesity effect, improving lipid metabolism and reducing hepatic enzyme activity. A significant reduction in plaque size and lipid content was observed in the aortic sinus of APH-fed mice, compared to the control (p < 0.001), whereas no differences in the extracellular matrix and macrophage recruitment were observed. Supplementation of APH significantly attenuates atherosclerosis in ApoE−/− mice, exerting a lipid-lowering activity. The opportunity to use fish waste protein hydrolysates as a nutraceutical in atherosclerosis is worthy of future investigations, representing a low cost, sustainable, and nutritional strategy with minimal environmental impact.
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CITATIONS (13)
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