Serological Survey and Molecular Characterization of Theileria annulata in Sicilian Cattle

• DOI: 10.3390/pathogens10020101
• Publication Date: 2021-01-21T16:49:40Z
• Authors: Valeria Gargano; Valeria Blanda; Delia Gambino; Francesco La Russa; Sophia Di Cataldo; Antonino Gentile; Giorgia Schirò; Alessandra Torina; Javier Millán; Domenico Vicari
• Keywords: Theileria; Rhipicephalus sanguineus; Hyalomma; Molecular Epidemiology; Sicilian

Tropical theileriosis is a tick-borne disease caused by hemoprotozoan parasites with considerable veterinary and economic impact worldwide. Ticks transmitting the belong to Haemaphysalis, Rhipicephalus, Hyalomma genera. The genus very common in Sicily (Italy) represents main Theileria annulata vector island. Data concerning molecular epidemiology of this pathogen are missing region. In 2018–2019, blood serum samples were collected from 480 cows seven Sicilian farms four different provinces. Seroprevalence ranged 22% 71%. Three selected for analysis consisting real-time PCR targeting almost complete 18S ribosomal RNA (rRNA). Four amplicons per farm sequenced phylogenetic analyses carried out. sequences identical within each showed 92–99% identity other Genbank. According analysis, these three an additional one laboratory-cultured strain obtained 1999 belonged single T. clade good bootstrap support Italy, India, Iran, indicating limited geographical temporal genetic variability parasite. This study first Sicily, which will be useful improve strategies control prevention.