Molecular Typing of Staphylococcus aureus Isolated from Patients with Autosomal Dominant Hyper IgE Syndrome

• DOI: 10.3390/pathogens6020023
• Publication Date: 2017-06-06T14:53:09Z
• Authors: Inka Sastalla; Kelli Williams; Erik Anderson; Ian Myles; Jensen Reckhow; Marlene Espinoza-...; Alexandra Freeman; Sandip Datta
• Keywords: Multilocus sequence typing; Primary Immunodeficiency; Leukocidin

Autosomal dominant hyper IgE syndrome (AD-HIES) is a primary immunodeficiency caused by loss-of-function mutation in the Signal Transducer and Activator of Transcription 3 (STAT3). This immune disorder clinically characterized increased susceptibility to cutaneous sinopulmonary infections, particular with Candida Staphylococcus aureus. It has recently been recognized that skin microbiome patients AD-HIES altered an overrepresentation certain Gram-negative bacteria Gram-positive staphylococci. However, these alterations have not at species- strain-level. Since S. aureus infections are influenced strain-specific expression virulence factors, information on colonizing strain characteristics may provide insights into host-pathogen interactions help guide management strategies for treatment prophylaxis. The aim this study was determine whether selects unique strains Using multi-locus sequence typing (MLST), protein A (spa) typing, PCR-based detection toxin genes, we performed detailed analysis isolates (n = 13) found twenty-one AD-HIES. We low diversity types, abundance expressed methicillin resistance, Panton-Valentine leukocidin (PVL), staphylococcal enterotoxins K Q (SEK, SEQ). Our results indicate often carry antibiotic-resistant harbor key factors.