Molecular Analysis of Canine Filaria and Its Wolbachia Endosymbionts in Domestic Dogs Collected from Two Animal University Hospitals in Bangkok Metropolitan Region, Thailand

• DOI: 10.3390/pathogens8030114
• Publication Date: 2019-07-29T15:20:18Z
• Authors: Hathaithip Satjaw...; Atchara Phumee; Sonthaya Tiawsirisup; Sivapong Sungpradit; Narisa Brownell; Padet Siriyasatien; Kanok Preativatanyou
• Keywords: 0301 basic medicine; R; zoonosis; Thailand; Article; 3. Good health; 03 medical and health sciences; dog; <i>B. pahangi</i>; Medicine; <i>D. immitis</i>; <i>B. malayi</i>; filariasis

Canine filariasis is caused by several nematode species, such as Dirofilaria immitis, repens, Brugia pahangi, malayi, and Acanthocheilonema reconditum. Zoonotic one of the world's neglected tropical diseases. Since 2000, World Health Organization (WHO) has promoted a global filarial eradication program to eliminate 2020. Apart from vector control strategies, infection reservoir hosts necessary for more effective control. In addition, many studies have reported that Wolbachia development, reproduction, survival nematode. Consequently, use antibiotics kill in nematodes now become an alternative strategy filariasis. Previously, case subconjunctival dirofilariasis spp. been woman who resides center Bangkok, Thailand. Therefore, our study aimed principally demonstrate presence bacteria blood collected domestic dogs Bangkok Metropolitan Region, A total 57 samples with suspected had visited veterinary clinics were collected. The investigations microfilaria carried out using both microscopic molecular examinations. PCR was used detection method based on COI ITS1 regions. demonstration performed amplify FtsZ gene. All positive then cloned sequenced. results showed detected 16 (28.07%) species COI-PCR revealed 50 (87.72%) positive; these consisted 33 (57.89%), 13 (22.81%), 4 (7.02%) D. B. respectively. While ITS1-PCR 41 (71.93%) positive-30 (52.63%) identified containing immitis 11 (19.30%) whereas malayi not detected. Forty-seven (82.45%) DNA phylogenetic tree all classified into supergroup C clade. This established fundamental data associated Region. An extensive survey dog would provide valuable epidemiologic potential zoonotic this information could be future development prevention strategies canine