Isolation, Characterization, and Application of a Bacteriophage Infecting the Fish Pathogen Aeromonas hydrophila

• DOI: 10.3390/pathogens9030215
• Publication Date: 2020-03-17T13:27:41Z
• Authors: Muhammad Akmal; Aryan Rahimi-Midani; Muhammad Hafeez-u...; Ali Hussain; Tae-Jin Choi
• Keywords: Siphoviridae

Bacteriophages are increasingly being used as biological control agents against pathogenic bacteria. In the present study, we isolate and characterize bacteriophage Akh-2 from Geoje Island, South Korea, to evaluate its utility in controlling motile Aeromonas septicemia. lysed four of seven hydrophila strains tested. Transmission electron microscopy analysis showed that belongs Siphoviridae family, with head tail sizes 50 ± 5 170 nm, respectively. One-step growth curve revealed phage has a latent period min burst size 139 plaque-forming units per infected cell. The appeared stable pH range 6-8 temperature -80 46 °C. Based on next-generation sequencing analysis, genome is 114,901 bp size, 44.22% G + C content 254 open reading frames. During an artificial induction disease, loach (Misgurnus anguillicaudatus) treated increased survival rate time compared non-treated control. Our results suggest potential agent for treatment infections fish.