Comparison of Coxiella burnetii Excretion between Sheep and Goats Naturally Infected with One Cattle-Associated Genotype

• DOI: 10.3390/pathogens9080652
• Publication Date: 2020-08-13T13:23:44Z
• Authors: Benjamin Bauer; Louise Prüfer; Mathias Walter; Isabel Ganter; Dimitrios Frangou...; Martin Runge; Martin Ganter
• Keywords: Coxiella burnetii; Q fever; Domestic sheep reproduction; Multilocus sequence typing

The main reservoir of Coxiella (C.) burnetii are ruminants. They shed the pathogen through birth products, vaginal mucus, faeces and milk. A direct comparison C. excretions between naturally infected sheep goats was performed on same farm to investigate species-specific differences. animals were vaccinated with an inactivated phase I vaccine at beginning study period for public health reasons. Vaginal rectal swabs along milk specimens taken monthly during lambing once again next season. To estimate environmental contamination animals’ housings, nasal from every animal simultaneously. Moreover, dust samples windowsills straw beddings collected. All examined by qPCR targeting IS1111 gene MLVA/VNTR typing method performed. Whole genome sequencing applied determine number copies followed a calculation equivalents each sample. cattle-associated genotype C7 detected containing 29 copies. Overall, seem more mucus in particular longer via route than sheep. This is supported larger quantities DNA caprine compared ovine ones. Transmission cattle small ruminants must also be considered.