Research on Reflective High-Speed Multi-Point Confocal Microscopy System

• DOI: 10.3390/photonics12010022
• Publication Date: 2024-12-31T12:51:57Z
• Authors: Huiqin Hu; Ning He; Kuangwei Du; Yukun Zhu; Dakai Zhou; Yuan Mi; Yunbo Chen; Junteng Ma; Youhua Chen; Cuifang Kuang
• Keywords: Microlens; Point spread function; Field of view; Bright-field microscopy

Reflective confocal imaging systems often face challenges in achieving high resolution, speed, and uniformity, particularly for reflective sample imaging. This study introduces a multi-point system designed to improve expand the field of view, optimize performance using incoherent, telecentric light-emitting diode (LED) illumination. By integrating uniform illumination with microlens arrays optimizing instantaneous structured microscopy optical components, achieves resolution 1116 lp/mm (20×, 0.42), 100 fps imaging, 500 µm × view while improving contrast. A comparative analysis laser- LED-based scanning demonstrates that incoherent provides superior uniformity reduces intensity variance Validation through photon-reassignment experiments, target nm metal particle visualization confirmed system’s enhanced resolution. Furthermore, ex vivo corneal revealed cellular-level details, suggesting its potential noncontact diagnostics.