Cytotoxic, Apoptotic and Genotoxic Effects of Lipid-Based and Polymeric Nano Micelles, an In Vitro Evaluation

• DOI: 10.3390/toxics6010007
• Publication Date: 2018-01-03T17:00:06Z
• Authors: Fatemeh Bahadori; Abdurrahim Kocyigit; Hayat Onyuksel; Aydan Dag; Gulacti Topcu
• Keywords: Acridine orange; Sulforhodamine B; Ethidium bromide

Self-assembly systems (SAS) mainly consist of micelles, and liposomes are the classes Nano Drug Delivery Systems with superior properties compared to traditional therapeutics in targeting cancer tumors. All commercially available nano-formulations chemotherapeutics currently SAS. According our knowledge, a specific toxicity comparison based on material differences has not yet been performed. The purpose this study was evaluate compare two SAS consisting Sterically Stabilized Micelles (SSM) made lipid-based amphiphilic distearoyl-sn-glycero-phosphatidylethanolamine-polyethylene glycol (PEG)-2000 polymeric micelle (PM) Y-shape block copolymer, synthesized using poly ε-caprolactone PEG. mechanism cytotoxicity genotoxicity micelles L-929 healthy mouse fibroblast cells assessed Sulforhodamine-B, WST-1, Acridine Orange/Ethidium Bromide alkaline single-cell gel electrophoresis assays. Results showed that SSM conc. 40 mg/mL shows very low at end 24, 48 72 h. DNA damage caused by much lower than PM while latter one significant causing apoptosis ED50 value 3 mg/mL. While ignorable, some chain breaks were detected treated PM.