Characterisation of adipocyte‐derived extracellular vesicles released pre‐ and post‐adipogenesis

• DOI: 10.3402/jev.v4.29159
• Publication Date: 2015-11-24T09:46:41Z
• Authors: Katherine D. Conn...; Irina A. Guschina; Vincent Yeung; Aled Clayton; Mohd Shazli Draman; Christopher Von R...; Marian Ludgate; Philip E. James; D. Aled Rees
• Keywords: Lipid droplet; Nanoparticle tracking analysis; Microvesicle

Extracellular vesicles (EVs) are submicron released from many cell types, including adipocytes. EVs implicated in the pathogenesis of obesity-driven cardiovascular disease, although characteristics adipocyte-derived not well described. We sought to define before and after adipogenesis, hypothesising that adipogenesis would affect EV structure, molecular composition function. Using 3T3-L1 cells, were harvested at day 0 15 differentiation. preparations visualised by electron microscopy quantified nanoparticle tracking analysis (NTA). then assessed for annexin V positivity using flow cytometry; lipid phospholipid 2D thin layer chromatography gas chromatography; vesicular protein content an immuno-phenotyping assay. Pre-adipogenic cells connected via a network protrusions both time points display classic morphology. concentration is elevated prior particularly exosomes small microvesicles. Parent contain higher proportions phosphatidylserine (PS) show binding. Both increased proportion arachidonic acid 0. PREF-1 was whilst adiponectin indicating reflects stage cell. Our data suggest production <300 nm. Cells this point possess greater PS (required generation) corresponding enriched signalling fatty acids, such as acid, markers PPARγ.