Synthesis of protein-coated biocompatible methotrexate-loaded PLA-PEG-PLA nanoparticles for breast cancer treatment

• DOI: 10.3402/nano.v7.31996
• Publication Date: 2016-06-16T09:33:05Z
• Authors: Salam Massadeh; Manal Alaamery; Shatha Al-Qatanani; Saqer Alarifi; Shahad Bawazeer; Yusra Alyafee
• Keywords: Biocompatibility; Vinyl alcohol; Zeta potential

BackgroundPLA-PEG-PLA triblock polymer nanoparticles are promising tools for targeted dug delivery. The main aim in designing polymeric drug delivery is achieving a controlled and release of specific at the therapeutically optimal rate choosing suitable preparation method to encapsulate efficiently, which depends mainly on nature (hydrophilic or hydrophobic). In this study, methotrexate (MTX)-loaded were prepared by double emulsion method.MethodBiodegradable polyethylene glycol-polylactide acid tri-block was used with poly(vinyl alcohol) as emulsifier. resulting coated bovine serum albumin order improve their biocompatibility. This study focused particle size distribution, zeta potential, encapsulation efficiency, loading capacity, vitro various concentrations PVA (0.5%, 1%, 2%, 3%).ResultsReduced methotrexate-loaded obtained using lower concentrations. Enhanced efficiency capacity 1% PVA. FT-IR characterization conducted void drug-loaded methotrexate, protein-coated solid state showed structure plain PEG-PLA methotrexate. PLA-PEG-PLA have been studied vitro; release, loading, yield reported.ConclusionThe profile monitored over period 168 hours, free burst effect before protein coating. results from work promising; can be taken further develop MTX based therapies.