Non-contact Dielectric Spectroscopy of Multi-Layered Substrates: Towards Organ-on-Chip Applications

• DOI: 10.36227/techrxiv.173834521.14976408/v1
• Publication Date: 2025-01-31T17:40:30Z
• Authors: Tim Hosman; Massimo Mastrangeli; Marco Spirito

Dielectric spectroscopy is a label-free, non-contact, real-time, multi-layer sensing technology, and has been used for identification quantification of many biological materials. A combination such features in demand monitoring organ-on-chip systems; however available technologies have yet to address this need. In work, we explore the possibility leveraging inherent dielectric application systems, by investigating three key technological developments using open-ended coaxial probes. Firstly, biocompatible non-contact capabilities are proved showing similar performance Parylene C-coated probes uncoated Secondly, setup methodology developed highly accurate non-destructive height positioning probe allow precise extraction intermediate sample layers. Finally, presented technology successfully demonstrated means phantom three-layered system. With further integration, can potentially become cornerstone technique enabling real-time tracking various tissue contents properties.