Cell Wall Proteins of Staphylococcus aureus Are Involved in Cross Reactivity with Murine Cytokine Assays

• DOI: 10.36348/sjpm.2021.v06i03.001
• Publication Date: 2021-06-26T01:57:22Z
• Authors: Mehak Gull; Abida Bano; Numan Javed
• Keywords: Cross-reactivity; Protein A

Staphylococcus aureus is one of the important pathogen often termed as superbug.After S. interacting with host innate immune system, it induces release pro-inflammatory cytokines like TNF-α and IL-1β from Immune cells.In current study, various strains their cell wall lysates indicated cross reactivity murine ELISA antibodies.In aureus, proteins are involved in this phenomenon.This was exhibited form unusual exaggerated signals while performing for quantification cytokines.The among were determined using SDS-PAGE.To all our data, revealed that unknown epitopes producing during by ELISA.This generated not only interaction bone marrow derived macrophages (BMDM) but also control settings where free system used ruling out phenomenon.