Suppression of gastric cancer growth by baculovirus vectormediated transfer of normal epithelial cell specific-1 gene

• DOI: 10.3748/wjg.14.5810
• Publication Date: 2008-10-31T10:01:51Z
• Authors: Wei Huang; Xiang-Long Tian; Yun-Lin Wu; Jie Zhong; Li-Fen Yu; Sheng-Ping Hu; Biao Li
• Keywords: Male; Mice, Inbred BALB C; Time Factors; Genetic Vectors; Green Fluorescent Proteins; Mice, Nude; Genetic Therapy; Transfection; Xenograft Model Antitumor Assays; 3. Good health; Mice; 03 medical and health sciences; 0302 clinical medicine; Genes, Reporter; Stomach Neoplasms; Transduction, Genetic; Cell Line, Tumor; Animals; Humans; Kallikreins; Baculoviridae; Cell Proliferation

To study the inhibitory effect of baculovirus-mediated normal epithelial cell specific-1 (NES1) gene therapy on gastric cancer (GC) in vitro and in vivo.We first constructed recombinant baculovirus vectors and then transfected them into gastric cancer cells (SGC-7901). Efficiency of the baculovirus for gene transfer into SGC-7901 cells and cell growth curves were detected by fluorescence microscopy, Western blot and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in vitro, respectively. The therapeutic effect of this gene therapy on GC was confirmed in xenografted nude mice. Tumor growth was determined by tumor volume, and expression of NES1 in tumor was analyzed by immunohistochemistry.Baculovirus vectors were successfully transfected into SGC-7901 cells. SGC-7901 cells transfected with the NES1 gene inhibited cell growth. In the Bac-NES1 treated group, tumor growth was significantly reduced with a high level of NES1 expressionBaculovirus-mediated NES1 gene can be used in gene therapy for GC.