Notch2 regulates matrix metallopeptidase 9viaPI3K/AKT signaling in human gastric carcinoma cell MKN-45

• DOI: 10.3748/wjg.v18.i48.7262
• Publication Date: 2013-01-13T10:30:39Z
• Authors: Ling-Yun Guo
• Keywords: HES1

To clarify the role of activated Notch2 in invasiveness gastric cancer.To investigate silencing gene expression, we established a small interfering RNA (siRNA) transfected cell line using MKN-45 cancer line. After successful transfection confirmed by real-time reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting, migration invasion assays were employed to evaluate aggressiveness cancer. RT-PCR blottings confirm down-regulation expression epithelial mesenchymal transition-related matrix metallopeptidase 9 (MMP9), Akt, p-Akt. relationship between PI3K-Akt MMP9, PI3K inhibitor LY294002 was used treat cells.Notch2 dramatically decreased after siRNA (100.00% ± 9.74% vs 11.61% 3.85%, P < 0.01 qRT-PCR). There also marked reduction Notch target Hes1 4.74% 61.61% 3.58%, 0.05) at mRNA, indicating an inhibition signaling. Inhibition signaling intracellular domain protein levels 65.61% 7.58%, 0.05). Down-regulation enhanced tumor 21.64% 162.22% 16.84%, MMP9 (1.56 fold, 0.05), pro-MMP9 its active form (1.48 no significant difference Akt two groups 10.87% 96.61% 7.33%, > while elevated p-Akt 9.87% 154.61% 13.10%, Furthermore, down-regulated response (p-Akt 100.00% 8.87% 58.27% 5.01%, 0.05; 9.17% 50.03% 4.88%, 0.05).Notch2 may negatively regulate inhibiting pathway