Preliminary exploration of animal models of congenital choledochal cysts

• DOI: 10.3748/wjg.v30.i10.1420
• Publication Date: 2024-03-14T12:50:24Z
• Authors: Shu-Hao Zhang; Yue-Bin Zhang; Duo-Te Cai; Tao Pan; Ken Chen; Yi Jin; Wen-Juan Luo; Zong-Wei Huang; Qing-Jiang Chen; Zhi-Gang Gao
• Keywords: Choledochal Cysts

BACKGROUND Various animal models have been used to explore the pathogenesis of choledochal cysts (CCs), but with little convincing results. Current surgical techniques can achieve satisfactory outcomes for treatment CCs. Consequently, recent studies focused more on clinical issues rather than basic research. Therefore, we need appropriate further AIM To establish an model that may contribute investigation METHODS Eighty-four specific pathogen-free female Sprague-Dawley rats were randomly allocated a group, sham or control group. A rat CC was established by partial ligation bile duct. The reliability confirmed measurements serum biochemical indices, morphology common ducts as well molecular biology experiments in and human tissues. RESULTS Dilation classified mild (diameter, ≥ 1 mm < 3 mm), moderate (≥ 10 severe mm) observed 17, 2 respectively, while no dilation groups. Serum levels alanine aminotransferase, aspartate total bilirubin, direct acids significantly elevated group compared 7 d after surgery, gamma-glutamyltransferase increased 14 surgery. Most indices gradually decreased normal ranges 28 protein expression trend signal transducer activator transcription consistent CONCLUSION duct juvenile could morphologically simulate cystic fusiform CC, which investigating CC.