Is activation of AMP-activated protein kinase involved in ropivacaine-induced SH-SY5Y cell apoptosis?

• DOI: 10.3760/cma.j.issn.0254-1416.2011.02.019
• Publication Date: 2011-02-20
• Authors: Zhou Shu-qin; Jun Lu; Qingguo Zhang; Rui Xu; Luying Lai; Shiyuan Xu
• Keywords: SH-SY5Y; Viability assay; AMP-Activated Protein Kinase; MTT assay

Objective To determine if activation of AMP-activated protein kinase (AMPK) is involved in ropivacaine-induced reactive oxygen species (ROS) production and apoptosis human neuroblastoma cell line SHSY5Y.Methods SH-SY5Y was purchased from center Shanghai life Science Research Institute,Chinese Academy Sciences cultured DMEM/F12 liquid culture medium containing 15 % bovine calf serum at 37 ℃ incubator filled with 5% CO2 . Plasmids pGPU6/GFP/Neo-shRNA AMPKα2 pEGFP-N1-AMPKα2were transfected into the line. The expression determined by Western blot analysis.The cells recombinant plasmids were exposed to 3 mol/L ropivacaine. Intracellular ROS detected flow cytometry. Cell viability quantitatively MTT colorimetry assay. Apoptosis assessed cytometry Hoechst33258 staining. Results plasmid pEGFP-N1-AMPKα2 upregulated while down-regulated ( P < 0.01). Down-regulation attenuated up-regulation of. promoted intracellular induced ropivacaine 0.01) Conclusion AMPK probably mediates

Key words: Protein kinases; Amides;