Study on the resistant genes and virulence gene of vancomycin-resistant Enterococci

• DOI: 10.3760/cma.j.issn.0254-5101.2012.09.002
• Publication Date: 2012-09-30
• Authors: Yamin Lu; Xiaolei Zhang; Jianming Cao
• Keywords: Teicoplanin; Multilocus sequence typing; Linezolid; Enterococcus faecium; Agar dilution; Virulence factor

Objective To study the vancomycin-resistant genes and virulence factors in Enterococci (VRE),and to analyze drug-resistance character epidemic characteristics of VRE strains provide basis for clincal selection drugs infection control.Methods were screened by agar dilution sieving plate (ADSP) containing 6 μg/ml vancomycin,drug resistance other common antibiotics detected VITEK-60 automatic microbial analyzer.The gene types factor determined PCR.And genetic relationships among multilocus sequence typing.Results Seven Enterococcus faecium found 360 enterococcus strains.All exhibited high-level vancomycin ; some them medium or senstive teicoplanin.They all carried vanA esp one 4 kinds genes.The ST type 7 diffused distribution.Conclusion We vanB phenotype genotype isolates Wenzhou; these multidrug various genes.Linezolid could be used as a recommend drug treatment infection.The protection sensitivity should strengthened.

Key words: Enterococci; Vancomycin-resistant Enterococci; Virulence gene; Multilocus typing