Negative association of donor age with CD34+ cell dose in mixture allografts of G-CSF-primed bone marrow and G-CSF-mobilized peripheral blood harvests

• DOI: 10.3760/cma.j.issn.0366-6999.20141161
• Publication Date: 2024-01-16T17:33:15Z
• Authors: Li Yan; Chang Yingjun; Xu Lanping; Zhang Xiaohui; Huang Xiaojun
• Keywords: Adult; Male; Adolescent; Antigens, CD34; Middle Aged; Allografts; Hematopoietic Stem Cell Mobilization; Tissue Donors; Young Adult; Bone Marrow; Granulocyte Colony-Stimulating Factor; Humans; Female; Prospective Studies; Aged; Bone Marrow Transplantation

Background The effects of donor characteristics on CD34 + cell dose remain controversial. Recently, we developed a novel haploidentical transplant protocol, in which mixture allografts granulocyte colony-stimulating factor (G-CSF)-primed bone marrow (G-BM) and G-CSF-mobilized peripheral blood (G-PB) were used. aim this study was to investigate the G-BM G-PB. Methods A total 162 healthy adult donors, who underwent harvest collection between January 2009 November 2010 Peking University People's Hospital, prospectively investigated. G-CSF administered subcutaneously at 5 μg/kg once day for 5–6 consecutive days. Bone stem cells harvested fourth fifth day, respectively. final less than 2×10 6 cells/kg recipient body weight considered poor mobilization. Results Of 31 (19.1%) did not attain threshold. obtained median doses marrow, blood, 0.83×10 /kg, 2.40×10 3.47×10 Multiple regression analysis showed that age had significant negative effect either G-BM, or G-PB, And 1-year increase associated with 5.6% decrease odds achieving mobilization cutoff. No correlation found gender, mass index (BMI), weight. Conclusion Donor is only among four parameters, including age, weight, BMI, influence younger donors should be chosen obtain sufficient transplantation.