Optimal timing of antenatal taurine supplementation for improvement of neuron and neural stem cell proliferation in fetal rats with intrauterine growth restriction

• DOI: 10.3760/cma.j.issn.1007-9408.2016.07.009
• Publication Date: 2016-07-16
• Authors: Lifang Zhao; Fang Li; Jing Liu; Huawei Wang
• Keywords: Intrauterine growth restriction; Litter; Group B; Group A; Fetal growth

Objective To determine the optimal timing of antenatal taurine supplementation to improve neuron and neural stem cell proliferation in fetal rats with intrauterine growth restriction.

Methods Twenty-five pregnant SD were randomly divided into five groups (five each group): group A was control group, B E restriction (FGR) model low-protein diet during experiment, C, D, supplemented [300 mg/(kg·d)] at day 9, 11 15, respectively. The birth weight newborn measured after natural delivery. body two standard deviations lower than average diagnosed as FGR. There litters selected from litter, resulting ten group. Proliferating nuclear antigen (PCNA) fatty acid binding protein 7 (FABP7) positive expression rat brain tissues detected by immunohistochemistry. Single factor analysis variance, LSD tests used for statistical analysis.

Results The A, B, D (6.61±0.45), (4.65±0.23), (5.37±0.17), (5.74±0.21), (5.00±0.24) g, Average (t=2.447), higher (t=2.306 2.306), C differences statistically significant (all P 0.05). IOD that E, difference (t=4.182, P<0.05).

Conclusions Antenatal can promote effect is most obvious on 11th pregnancy, may lead promotion development.

Key words: Fetal retardation; Taurine; Neurons; Neural cells; Cell proliferation; Proliferating antigen; Nerve tissue proteins; Fatty acid-binding proteins; Disease models, animal